CD80 Antibody

• Catalog Number: orb1239276
• Category: Antibodies
• Description: CD80 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 11D1
• Tested applications: ELISA, FC, ICC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG1
• Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.
• Concentration: 1 mg/mL
• Dilution range: CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 50 kDa
• Target: CD80
• UniProt ID: P33681
• NCBI: NP_005182
• Storage: CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1,
• Note: For research use only
• Application notes: CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL. Red: CD80 Antibody [11D1] (orb1239276) Blue: DAPI staining

Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL. Red: CD80 Antibody [11D1] (orb1239276) Blue: DAPI staining

Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL. Red: CD80 Antibody [11D1] (orb1239276) Blue: DAPI staining

Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.

Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.

Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.