CD80 Antibody

SKU: orb1240009

Description

CD80 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit
• Application Notes:
  CD80 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: CD80 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human CD80.The immunogen is located within amino acids 60 - 110 of CD80.
• Target: CD80
• Molecular Weight: Predicted: 32 kDa Observed: 50 kDa
• Purification: CD80 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1

Western blot analysis of CD80 in EL4 cell lysate with CD80 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 20 µg/ml. Red: CD80 Antibody (orb1240009) Blue: DAPI staining.

Quick Database Links

Gene Symbol

CD80

UniProt

P33681

RefSeq (Protein):NP_005182

Roham Deyhimfar 1, Keykavos Gholami 2, Iman Menbari Oskouie 1, Leila Zareian Baghdadabad 1, Parisa Zahmatkesh 1, Mohammadreza Shoghi 3, Gholamreza Mesbah 4, Fateme Guitynavard 1, Seyed Mohammad Kazem Aghamir 5 High-dose vitamin C improves BCG immunotherapy's efficacy in a murine ectopic model of bladder cancer Pathol Res Pract, (2025)

PubMed: 40907423

Applications

IHC

Reactivity

Mouse