Cytokeratin 8/KRT8 Mouse Monoclonal Antibody

SKU: orb3085265

Description

The Cytokeratin 8 KRT8 Mouse Monoclonal Antibody (Carrier-free) is a Carrier-free antibody raised in mouse. It is immunogen affinity purified and suitable for use in FC, ICC, IF, IHC, IHC-Fr, and WB applications. This antibody targets the Keratin, type II cytoskeletal 8 in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Immunology & Inflammation, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunofluorescence, 2μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: B5I1
• Immunogen: E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.
• Target: Keratin, type II cytoskeletal 8
• Molecular Weight: 54 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CARD2; Cell and organelle markers; CK 8; CK8; CYK8; Cytokeratin 8; Cytoskeleton Marker; K2C8; K8; keratin 8; KO; KRT8; Type II keratin Kb8

Quick Database Links

Gene Symbol

Keratin, type II cytoskeletal 8

UniProt

P05787