CD75 Rabbit Polyclonal Antibody

SKU: orb7144

Description

CD75 Rabbit Polyclonal Antibody

Research Area

CD Markers, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD75 (301-406/406aa)
• Target: ST6GAL1
• Molecular Weight: 47 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

6 sialyltransferase; 6-sialyltransferase 1; 6-ST 1; alpha 2; alpha 2, 6 ST 1; alpha 2, 6 ST; B cell antigen CD75; B-cell antigen CD75; beta galactoside alpha 2, 6 sialyltransferase 1; beta-galactoside alpha-2; CMP N acetylneuraminate beta galactosamide alpha 2, 6 sialyltransferase 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; MGC48859; Sialyltransferase 1(beta galactoside alpha 2, 6 sialyltransferase); Sialyltransferase 1; SIAT1; SIAT1_HUMAN; ST6 beta galactosamide alpha 2, 6 sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N.

25 ug total protein per lane of various lysates probed with CD75 polyclonal antibody, unconjugated (orb7144) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-CD75 (orb7144) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48 kD.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, Primary: Anti-CD75 (orb7144) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48 kD.

Quick Database Links

Gene Symbol

ST6GAL1

UniProt

P15907