CD74 / CLIP Antibody

• Catalog Number: orb2639873
• Category: Antibodies
• Description: Recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74/CLIP. Its epitope is localized in the extracellular domain of CD74. CD74/CLIP is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74/CLIP is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. It is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74/CLIP stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas and has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CLIP/813
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CLIP antibody. Its epitope is localized in the extracellular domain of CLIP/CD74.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells (methanol-fixed),Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CLIP antibody is available for research use only.
• UniProt ID: P04233
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the ACTH / POMC antibody for each application should be determined by the researcher.1. View the recombinant version of this ACTH antibody.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Raji cell lysate with CLIP antibody (clone CLIP/813). Expected molecular weight: 33-43 kDa.

Immunofluorescent staining of human Raji cells with CLIP antibody (green, clone CLIP/813) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with CLIP antibody (clone CLIP/813); Red = isotype control, Blue = CLIP antibody.

IHC analysis of formalin/paraffin human tonsil with CLIP antibody (clone CLIP/813).