CD74 / CLIP Antibody

• Catalog Number: orb2637624
• Category: Antibodies
• Description: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LN-2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: SU-DHL-4 lymphoma cells were used as the immunogen for this CD74 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence (methanol fixed): 1-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD74 antibody is available for research use only.
• Entrez: 972
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Raji cells with CD74 antibody (clone LN-2, green) and Reddot nuclear stain (red).

IHC staining of FFPE human spleen tissue with CD74 antibody (clone LN-2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with CD74 antibody (clone LN-2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Raji cell lysate with CD74 antibody (clone LN-2). Expected molecular weight: 33-43 kDa.

Flow cytometry testing of human Raji cells with CD74 antibody (clone LN-2); Red = isotype control, Blue = CD74 antibody.