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CD73 Antibody

Catalog Number: orb2642916

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2642916
CategoryAntibodies
DescriptionCD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
Species/HostMouse
ClonalityMonoclonal
Clone NumberNT5E/2545
Tested applicationsELISA, FACS, IF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenA recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis CD73 antibody is available for research use only.
UniProt IDP21589
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the CD73 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
CD73 Antibody

IHC staining of FFPE human tonsil tissue with CD73 antibody (clone NT5E/2545). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

CD73 Antibody

IHC staining of FFPE human tonsil tissue with CD73 antibody (clone NT5E/2545). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

CD73 Antibody

IHC staining of FFPE human prostate carcinoma with CD73 antibody (clone NT5E/2545). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

CD73 Antibody

IHC staining of FFPE human pancreatic carcinoma with CD73 antibody (clone NT5E/2545). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

CD73 Antibody

IHC staining of FFPE human colon carcinoma with CD73 antibody (clone NT5E/2545). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

CD73 Antibody

Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone NT5E/2545); Red=isotype control, Blue= CD73 antibody.

CD73 Antibody

Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone NT5E/2545) and Reddot nuclear stain (red).

CD73 Antibody

Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone NT5E/2545). Predicted molecular weight: 60-70 kDa.

CD73 Antibody

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD73 antibody (clone NT5E/2545). These results demonstrate the foremost specificity of the NT5E/2545 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

CD73 Antibody

SDS-PAGE analysis of purified, BSA-free CD73 antibody (clone NT5E/2545) as confirmation of integrity and purity.

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