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Catalog Number | orb1519866 |
---|---|
Category | Antibodies |
Description | CD73 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | ICC, IHC, WB |
Reactivity | Human |
Immunogen | residues 27-552 (ECD) |
Concentration | 100 µg/ml |
Dilution range | WB - 1:1000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CD73 |
UniProt ID | P21589 |
NCBI | NP_002517.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | eN; NTE; NT5; CALJA; NT; Purine 5-Prime-Nucleotida Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human CD73 by western blot. Samples: Whole cell lysate (50 µg) from NCI-H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519866) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal.
Detection of human CD73 by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519866) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human CD73 by immunocytochemistry. Sample: FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519866) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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