NT5E Antibody

• Catalog Number: orb1265162
• Category: Antibodies
• Description: NT5E Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 63 kDa
• Target: NT5E
• UniProt ID: P21589
• NCBI: P21589
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of lysates from U266B1, HepG2 cell line, mouse brain tissue lysate (from left to right), using NT5E-M535 Antibody at 1:2000 at each lane. Lysates at 20ug per lane.

Western blot analysis of NT5E using rabbit polyclonal NT5E Antibody (M535) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.

Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Western blot analysis of CD73 Pab in mouse brain tissue lysate