CD73 Antibody

• Catalog Number: orb1519864
• Category: Antibodies
• Description: CD73 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, WB
• Reactivity: Human
• Immunogen: residues 27-552 (ECD)
• Concentration: 100 µg/ml
• Dilution range: WB - 1:1000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD73
• UniProt ID: P21589
• NCBI: NP_002517.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: eN; NTE; NT5; CALJA; NT; Purine 5-Prime-Nucleotida
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD73 by western blot. Samples: Whole cell lysate (50 µg) from H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519864) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal.

Detection of human CD73 by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519864) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human CD73 by immunocytochemistry. Sample: FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (orb1519864) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.