CD69 Rabbit Polyclonal Antibody

• Catalog Number: orb500673
• Category: Antibodies
• Description: CD69 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Canine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD69 (101-199/199aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27-33 kDa
• Target: CD69
• UniProt ID: Q07108
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CLEC2C; AIM; Early activation antigen CD69; Early
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Sample: Lane 1: Blood cell (Mouse) Lysate at 40 ug, Lane 2: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD69 (orb500673) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33/27 kD, Observed band size: 30 kD.

Sample: U937 Cell Lysate at 30 ug, Primary: Anti-CD69 (orb500673) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 34 kD.