CD68 Rabbit Polyclonal Antibody

SKU: orb317598

Description

CD68 Rabbit Polyclonal Antibody

Research Area

Innate Immunity, Myeloid Cells, SARS /COVID-19

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Mouse, Rat
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD68 (151-250/335aa)
• Target: CD68
• Molecular Weight: 37 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68(microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP4.

Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-CD68 antibody (orb317598), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

OCT embedded (Rat spleen), acetone-fixed, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (orb317598) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-CD68 (orb317598) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 110 kD.

Quick Database Links

Gene Symbol

CD68

UniProt

P31996