CD68 Rabbit Polyclonal Antibody

• Catalog Number: orb317598
• Category: Antibodies
• Description: CD68 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD68 (151-250/335aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: CD68
• UniProt ID: P31996
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-CD68 antibody (orb317598), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

OCT embedded (Rat spleen), acetone-fixed, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (orb317598) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-CD68 (orb317598) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 110 kD.