CD68 Rabbit Polyclonal Antibody

SKU: orb1499422

Description

CD68 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, FC
• Dilution range: Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant mouse CD68 protein (119-298/326aa)
• Target: CD68
• Molecular Weight: 110-140 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68(microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP

Blank control (black line): Raw264.7, Primary Antibody (green line): Rabbit Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): Raw264.7, Primary Antibody (green line): Rabbit Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Mouse Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Mouse Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD68 Antibody, the EC50 is 142.5 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD68 Antibody, the EC50 is 175.4 ng/ml.

Quick Database Links

Gene Symbol

CD68

UniProt

P31996