CD68 Rabbit Polyclonal Antibody

• Catalog Number: orb1499422
• Category: Antibodies
• Description: CD68 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant mouse CD68 protein (119-298/326aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110-140 kDa
• Target: CD68
• UniProt ID: P31996
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Raw264.7, Primary Antibody (green line): Rabbit Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): Raw264.7, Primary Antibody (green line): Rabbit Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Mouse Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Mouse Anti-CD68 antibody (orb1499422), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD68 Antibody, the EC50 is 142.5 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD68 Antibody, the EC50 is 175.4 ng/ml.