CD63 Antibody / LAMP-3

• Catalog Number: orb2637613
• Category: Antibodies
• Description: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MX-49.129.5
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length human CD63 was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD63 antibody is available for research use only.
• Entrez: 967
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE prostate carcinoma with CD63 antibody (clone MX-49.129.5).

IHC testing of FFPE human melanoma stained with CD63 antibody (clone MX49.129.5).

IHC testing of FFPE mouse spleen stained with CD63 antibody (clone MX49.129.5).

FACS testing of human PBMC: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate

FACS testing of mouse NIH3T3: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate

Western blot testing of human spleen lysate with CD63 antibody at 2 ug/ml (clone MX-49.129.5).

Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).

FACS staining of human MCF7 cells: Gray = unstained, Green = CF488 labeled CD63 antibody.

FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray = unstained, Green = CF488 labeled CD63 antibody.

FACS staining of human MCF7 cells: Gray = unstained, Orange = CF568 labeled CD63 antibody.

FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray = unstained, Orange = CF568 labeled CD63 antibody.