CD63 Antibody / LAMP-3

• Catalog Number: orb2637610
• Category: Antibodies
• Description: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NKI/C3
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this CD63 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G purified antibody
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD63 antibody is available for research use only.
• Entrez: 967
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human melanoma stained with CD63 antibody (NKI/C3).

IHC testing of mouse spleen stained with CD63 antibody (NKI/C3).

FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate

Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).

Western blot testing of human spleen lysate with CD63 antibody (clone NKI/C3).

Flow cytometry staining of live human MCF7 cells with biotin labeled CD63 antibody (clone NKI/C3) and streptavidin-CF568 (orange). Gray histogram represents unstained cells.

Flow cytometry staining of PFA-fixed human U-87 MG cells with CD63 antibody (clone NKI/C3); Red = isotype control, Blue = CD63 antibody.