CD59 antibody

• Catalog Number: orb44303
• Category: Antibodies
• Description: Mouse Monoclonal to CD59.
• Clonality: Monoclonal
• Clone Number: MEM-43/5
• Tested applications: FC, IHC-P, IP, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2b
• Immunogen: Thymocytes and T lymphocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD59
• Entrez: 966
• UniProt ID: P13987
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD59 antibody, anti-Protectin antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate; Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. orb44294)

Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).

Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).