CD59 glycoprotein CD59 Antibody

• Catalog Number: orb308756
• Category: Antibodies
• Description: CD59 glycoprotein CD59 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD59 recombinant protein (Position: L26-N102). Human CD59 shares 47.1% amino acid (aa) sequence identity with rat CD59.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25 μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 14177 MW
• UniProt ID: P13987
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD59 glycoprotein;1F5 antigen;20 kDa homologous re
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-CD59 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD59 using anti-CD59 antibody.Lane 1:human HeLa cell; 2:human PC-3 cell.

IF analysis of CD59 using anti-CD59 antibody. CD59 was detected in an immunocytochemical section of A431 cells.

IHC analysis of CD59 using anti-CD59 antibody. CD59 was detected in a paraffin-embedded section of human tonsil tissue.