CD59 Antibody

• Catalog Number: orb1671623
• Category: Antibodies
• Description: CD59 Antibody
• Clonality: Recombinant
• Clone Number: 193-27
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD59
• UniProt ID: P13987
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: 1F5, 1F5 antigen, CD59 glycoprotein, 20 kDa homolo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric r version of 193-27 (orb1671623) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671623- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the anti-CD59 antibody 193-27. Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of 193-27 (orb1671623 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.