CD58 antibody ()

• Catalog Number: orb1480522
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD58
• Clonality: Monoclonal
• Clone Number: MEM-63
• Tested applications: FC
• Reactivity: Human, Porcine
• Isotype: Mouse IgG1
• Immunogen: NALM-6 human pre-B cell line
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 µg/ml. Cold-induced transient opalescent turbidity may appear during the storage at 2 - 8°C. Bring the antibody to room temperature until the turbidity disappears, and mix well before the use.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD58
• Entrez: 965
• UniProt ID: P19256
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 8.8, 15 mM sodium azide
• Alternative names: LFA3, AG3
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml. Cold-induced transient opalescent turbidity may appear during the storage at 2 - 8°C. Bring the antibody to room temperature until the turbidity disappears, and mix well before the use.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from human CD58 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD58 (MEM-63) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD58 (MEM-63) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).