CD5 Antibody

• Catalog Number: orb443097
• Category: Antibodies
• Description: CD5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CD5 recombinant protein (Position: R25-L495).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 67 kDa
• UniProt ID: P06127
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: T-cell surface glycoprotein CD5; Lymphocyte antige
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of EL-4 cells using anti-CD5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD5 using anti-CD5 antibody.Lane 1:human Jurkat cell;2:human CCRF-CEM cell;3:mouse NIH3T3 cell.

IF analysis of CD68 and CD5 using anti-CD68 antibody and anti-CD5 antibody.CD68 and CD5 was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of CD5 using anti-CD5 antibody.CD5 was detected in paraffin-embedded section of mouse spleen tissue.

IHC analysis of CD5 using anti-CD5 antibody.CD5 was detected in paraffin-embedded section of human tonsil tissue.

IHC analysis of CD5 using anti-CD5 antibody.CD5 was detected in paraffin-embedded section of rat spleen tissues.