CD5 antibody

• Catalog Number: orb154398
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD5 which also designated Lyt-1 that has been identified as a transmembrane glycoprotein. It plays role as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2, as well as it is expressed at various developmental and activation stages on human B cells.
• Clonality: Monoclonal
• Clone Number: L17F12
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: Human acute lymphoblastic leukemia (ALL) T cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: Laurylmaltoside lysing buffer; non-reducing conditions; recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD5
• Entrez: 921
• UniProt ID: P06127
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-T1 antibody, anti-LEU1 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (L17F12) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (L17F12) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD5 (L17F12), 10 μg/ml.

Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.