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Description
Research Area
Immunology & Inflammation; Cell Biology
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD47, Fc Tag at 2 μg/ml can bind Human SIRP alpha, His Tag with a linear range of 16-250 ng/mL. |
| Target | CD47 |
| Tag | C-Fc Tag |
| MW | 40.4 kDa |
| Purity | >95% as determined by SDS-PAGE and more than 90% measured by SEC-HPLC. |
| Protein Sequence | Gln 19 - Pro 139 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD47, MER6, IAP, OA3
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Quality Guarantee
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The purity of rh CD47 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
CD47 Recombinant Protein (orb1228132)
- 0.0
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