CD47 Rabbit Polyclonal Antibody

SKU: orb526585

Description

CD47 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD47 (19-119/323aa)
• Target: CD47
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IAP; MER6; OA3; CD47_HUMAN; CD47; Antigenic surface determinant protein OA3; Integrin-associated protein (IAP); Protein MER6; CD47 molecule; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); antigen identified by monoclonal antibody 1D8; integrin associated protein; Rh-related antigen; leukocyte surface antigen CD47; CD47 glycoprotein

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-CD47 (orb526585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.

Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD47 (orb526585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722