CD47 Rabbit Polyclonal Antibody

• Catalog Number: orb526585
• Category: Antibodies
• Description: CD47 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD47 (19-119/323aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: CD47
• UniProt ID: Q08722
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Antigenic surface determinant protein OA3; CD 47;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-CD47 (orb526585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.

Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD47 (orb526585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 50 kD.