CD47 Antibody

• Catalog Number: orb1252798
• Category: Antibodies
• Description: CD47 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B6H12.2
• Tested applications: Blocking, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Intact CD47 purified from placenta was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Functional Activity (Blocking): Order BSA/Azide-free formatFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTWestern blot: 2-5 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD47
• UniProt ID: Q08722
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Leukocyte surface antigen CD47, Antigenic surface
• Note: For research use only
• Application notes: Functional Activity (Blocking): Order BSA/Azide-free formatFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTWestern blot: 2-5 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.