CD47 Antibody

SKU: orb1805751

Description

Anti-CD47 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Target: CD47
• Source/Expression System: CHO
• Molecular Weight: 145.58 kDa
• Purity: >95%
• Purification: Protein A
• Endotoxin: <0.001 EU/ug

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Concentration: Batch dependent
• Disclaimer: For research use only

Anti-CD47 Reference Antibody (lemzoparlimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CD47 Reference Antibody (lemzoparlimab)is more than 100% ;determined by SEC-HPLC.

Immobilized Recomvinant human CD47(c His) at1.5 μg/mL can bind Anti-CD47 Reference Antibody (lemzoparlimab);EC50=0.0163μg/mL.

PANC-1 cells were stained with Anti-CD47 Reference Antibody (lemzoparlimab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC317=0.265 ug/mL.

Anti-CD47 Reference Antibody (lemzoparlimab) FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 0.4557 ug/mL.

The phagocytosis ratio lemzoparlimab by CCRF-CEM increased with the increase of antibody concentration; and the phagocytosis Rate (%) reached 43%.

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722