Anti-CD47 Reference Antibody

• Catalog Number: orb1805751
• Category: Antibodies
• Description: Anti-CD47 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 145.5 kDa
• Target: CD47
• Endotoxins: < 1 EU/mg
• Storage: -80°C
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD47 Reference Antibody (lemzoparlimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CD47 Reference Antibody (lemzoparlimab)is more than 100% ;determined by SEC-HPLC.

Immobilized Recomvinant human CD47(c His) at1.5 μg/mL can bind Anti-CD47 Reference Antibody (lemzoparlimab);EC50=0.0163μg/mL.

PANC-1 cells were stained with Anti-CD47 Reference Antibody (lemzoparlimab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC317=0.265 ug/mL.

Anti-CD47 Reference Antibody (lemzoparlimab) FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 0.4557 ug/mL.

The phagocytosis ratio lemzoparlimab by CCRF-CEM increased with the increase of antibody concentration; and the phagocytosis Rate (%) reached 43%.