CD46 Rabbit Polyclonal Antibody

• Catalog Number: orb10329
• Category: Antibodies
• Description: CD46 Rabbit Polyclonal Antibody
• Target: CD46
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CD46 (251-355/355aa)
• UniProt ID: P15529
• RRID: AB_10751693
• MW: 43 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD46 antigen; CD46 molecule; CD46 molecule complem
• Research Area: Cell Markers, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: 293T (blue). Primary Antibody: Rabbit Anti-CD46 antibody (orb10329), Dilution: 1 µg in 100 1 µl 1X PBS containing 0.5% BSA (green), Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. protocol, The cells were washed twice with phosphate-buffered saline (PBS). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (orb10329, 1 µg/1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD46 antibody (orb10329), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10329, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD46) Polyclonal Antibody, Unconjugated (orb10329) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Epididymis (Mouse) Lysate at 40 ug, Primary: Anti-CD46 (orb10329) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 56 kD.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Human K562 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human MOLT4 cell lysates, Lane 6: Human MCF-7 cell lysates, Lane 7: Human Daudi cell lysates, Primary: Anti-CD46 (orb10329) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 52 kDa.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-CD46 (orb10329) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 55 kD.