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Catalog Number | orb44554 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to CD45R0. |
Clonality | Monoclonal |
Clone Number | UCHL1 |
Tested applications | FC, IHC-Fr, IHC-P, IP |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Human IL-2 dependent T cells |
Concentration | 1 mg/ml |
Dilution range | Immunohistochemistry (paraffin sections): This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Positive tissue: tonsil. Immunohistochemistry (frozen sections): Positive tissue: tonsil. Flow cytometry: Recommended dilution: 10 μg/ml. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD45R0 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CD45R0 antibody Read more... |
Note | For research use only |
Application notes | Immunohistochemistry (paraffin sections): This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Positive tissue: tonsil. Immunohistochemistry (frozen sections): Positive tissue: tonsil. Flow cytometry: Recommended dilution: 10 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD45R0 positive lymphocytes (red-filled) from CD45R0 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD45R0 (UCHL1) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45R0 (UCHL1) purified antibody (concentration in sample 1 μg/ml, GAM APC).
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