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CD45 Antibody Cocktail (Leukocyte marker)

Catalog Number: orb2638179

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2638179
CategoryAntibodies
DescriptionCD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation.Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. CD45RA contains the A exon and is a naive T-cell marker which may help prevent autoimmune disease. CD45RB contains B and stains most leukemias and lymphomas. CD45RC contains C and stains thymocytes, monocytes and dendritic cells. CD45RO doesn't contain A, B or C and is a marker of activated T-cells that can be used to classify and diagnose and classify lymphomas. This antibody cocktail will bind to all CD45 isoforms. The variation in these isoforms is localized to the extracellular domain, with the intracellular domain being conserved. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Species/HostMouse
ClonalityMonoclonal
Clone NumberSPM569 + SPM570
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenHuman B- and T-lymphoma cells were used as the immunogen for the CD45 antibody cocktail.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis CD45 antibody cocktail is available for research use only.
UniProt IDP08575
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesDue to variation in protocol and secondary antibody used, the CD45 antibody cocktail may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Expiration Date12 months from date of receipt.
CD45 Antibody Cocktail (Leukocyte marker)

IHC test of FFPE human tonsil probed with CD45 antibody cocktail

CD45 Antibody Cocktail (Leukocyte marker)

Western blot testing of human spleen lysate with CD45 antibody cocktail (clones SPM569 + SPM570). Expected molecular weight: 147-220 kDa depending on glycosylation level.

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