CD44V6/HCAM Rabbit Polyclonal Antibody

SKU: orb499620

Description

CD44V6/HCAM Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat CD44V6/HCAM (161-250/503aa)
• Target: CD44
• Molecular Weight: 53 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Homing Cell Adhesion Molecule; Epican; Heparan sulfate proteoglycan; Hyaluronate receptor; Antigen gp90 homing receptor; CD44; CD44 antigen; CD44 antigen homing function and Indian blood group system; CD44 molecule; CD44 molecule Indian blood group; CD44R; CDw44; CDW44 antigen; Chondroitin sulfate proteoglycan 8; CSPG8; ECMR III; ECMRIII; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; Hematopoietic cell E and L selectin ligand; Hermes antigen; Homing function and Indian blood group system; HUTCH I; IN; INLU related p80 glycoprotein; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH I; MUTCHI; PGP 1; Phagocytic glycoprotein I; CD44_RAT.

Blank control: Mouse spleen (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD44V6/HCAM antibody (orb499620, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CD44V6/HCAM antibody (orb499620), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

CD44

UniProt

P26051