CD44v4 Rabbit Polyclonal Antibody

• Catalog Number: orb5801
• Category: Antibodies
• Description: CD44v4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC
• Predicted Reactivity: Canine, Equine, Rabbit
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD44v4 (141-240/361aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:100, Flow-Cyt=1μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: CD44
• UniProt ID: P16070
• RRID: AB_10935103
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD44 antigen; CD44 antigen isoform 4 precursor; CD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4°C). Primary Antibody: Rabbit Anti-CD44v4 antibody (orb5801, Green), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: HELA. Primary Antibody (green line): Rabbit Anti-CD44v4 antibody (orb5801), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (orb5801) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (orb5801) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.