CD44v10 Rabbit Polyclonal Antibody

SKU: orb5800

Description

CD44v10 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD44v10 (218-223+472-477/742, 223=T -> Naa)
• Target: CD44
• Molecular Weight: 52/82 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD44v10 antibody (orb5800), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Lane 3: A549 (Human) Cell Lysate at 30 ug, Lane 4: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-CD44v10 (orb5800) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110-180 kD, Observed band size: 110-180 kD.

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

References

Aoshiba, Kazutetsu et al. Senescence-associated secretory phenotype in a mouse model of bleomycin-induced lung injury Exp Toxicol Pathol, 65, 1053-1062 (2013)

PubMed: 23688655

Applications

IHC

Reactivity

Mouse

Tei, Hiromoto et al. Expression profile of CD44s, CD44v6, and CD44v10 in localized prostate cancer: effect on prognostic outcomes following radical prostatectomy Urol Oncol, 32, 694-700 (2014)

PubMed: 24468288

Applications

IHC

Reactivity

Mouse