CD43 antibody

• Catalog Number: orb758925
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD43
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 84-3C1
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD43
• UniProt ID: P16150
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leukosialin; Sialophorin; GPL115; Galactoglycoprot
• Note: For research use only
• Application notes: This antibody can be used in the identification of malignant lymphomas. CD43 is expressed on 22-37% of B-cell lymphomas and, at the same time, it is not expressed on reactive B-cells. Consequently, B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma. If used with an anti-CD20 antibody, this clone may be utilized in the immunophenotyping of the lymphomas in formalin-fixed tissues, as Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 usually indicates lymphoma. Importantly, 84-3C1 antibody can be used for the detection of CD43 via various methods. For instance, it was successful in indetifying that antigen in a lysate of dendritic cells' platellet via Western blot (Julien et al., 2007). Finally, another group used this antibody during flow cytometric analysis a sample of B-cells (Yoon et al., 2017).
• Expiration Date: 12 months from date of receipt.