CD43 antibody

• Catalog Number: orb44350
• Category: Antibodies
• Description: Mouse Monoclonal to CD43.
• Clonality: Monoclonal
• Clone Number: MEM-59
• Tested applications: FC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human T lymphocytes.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml. Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil; recommended dilution: 10 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD43
• Entrez: 6693
• UniProt ID: P16150
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD43 antibody, anti-Leukosialin antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml. Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil; recommended dilution: 10 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59). Lane 1: lysate of human PBL; Lane 2: lysate of canine PBL; Lane 3: lysate of porcine PBL.

Separation of human CD43 positive lymphocytes (red-filled) from human CD43 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD43 (MEM-59) purified antibody (concentration in sample 2 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD43 (MEM-59) purified antibody (concentration in sample 2 µg/ml, GAM APC).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).