CD4 antibody (PE)

• Catalog Number: orb179883
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD4 conjugated to PE
• Clonality: Monoclonal
• Clone Number: OX-35
• Tested applications: FC
• Reactivity: Rat
• Isotype: Mouse IgG2a kappa
• Immunogen: MLR generated rat Th cells
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD4
• Entrez: 24932
• UniProt ID: P05540
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 4 antibody (PE), anti CD4 (L3T4) antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of rat splenocyte suspension stained using anti-rat CD4 (OX-35) PE antibody (concentration in sample 5 μg/ml).

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD4 (OX-35) PE antibody (concentration in sample 5 μg/ml).