CD294 Antibody (PE)

• Catalog Number: orb1570905
• Category: Antibodies
• Description: CD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.
• Clonality: Monoclonal
• Clone Number: BM16
• Tested applications: FC
• Reactivity: Human
• Isotype: Rat IgG2a
• Immunogen: Human CD294 transfected rat cell line TART/B19-12.10
• Conjugation: PE
• Target: CD294
• Entrez: 11251
• UniProt ID: Q9Y5Y4
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: DP2, PTGDR2, DL1R, CRTH2, GPR44
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human gated lymphocytes and basophils stained using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD294 (BM16) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD294 positive basophils (red-filled) from CD3 positive CD294 negative T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD294 (BM16) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD294 (BM16) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).