CD4 Antibody

SKU: orb153337

Description

Goat polyclonal antibody to CD4. CD4 is a member of the immunoglobulin superfamily, a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and a receptor for the human immunodeficiency virus. This protein is expressed not only on the surface of T helper cells and regulatory T cells but also on monocytes, macrophages and dendritic cells. It functions to initiate or amplify the early phase of T-cell activation and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC-Fr, IHC-P, WB
• Dilution Range: WB:1:500-1:2,000; IHC (F): 1:250-1:1,000; IHC (P): 1:250-1:1,000
• Reactivity: Canine, Human, Monkey, Mouse, Rat
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide derived from within 50 to 235 aa, corresponding to the external domain of human CD4 produced in E. coli. Antigen Sequence: FHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSKLTGSG
• Target: CD4 molecule
• Purity: This antibody is epitope-affinity purified from goat antiserum.
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
• Form/Appearance: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Concentration: Please inquire
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD4mut, CD4 antigen (p55), CD4 Receptor, T-cell surface antigen T4/Leu-3 and T-cell surface glycoprotein CD4 antibody.

IHC of mouse liver using anti-CD4 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD4 Ab at 1:750/DAB detection.

Western blot analysis of spleen cell line lysate using CD4 antibody.

Quick Database Links

Gene Symbol

CD4 molecule