CD4 antibody

• Catalog Number: orb153337
• Category: Antibodies
• Description: Goat polyclonal antibody to CD4. CD4 is a member of the immunoglobulin superfamily, a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and a receptor for the human immunodeficiency virus. This protein is expressed not only on the surface of T helper cells and regulatory T cells but also on monocytes, macrophages and dendritic cells. It functions to initiate or amplify the early phase of T-cell activation and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC-Fr, IHC-P, WB
• Reactivity: Canine, Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: Purified recombinant peptide derived from within 50 to 235 aa, corresponding to the external domain of human CD4 produced in E. coli.
• Concentration: 3 mg/ml
• Dilution range: WB:1:500-1:2,000; IHC (F): 1:250-1:1,000; IHC (P): 1:250-1:1,000
• Form/Appearance: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Purity: This antibody is epitope-affinity purified from goat antiserum.
• Conjugation: Unconjugated
• Target: CD4
• Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: CD4mut, CD4 antigen (p55), CD4 Receptor, T-cell su
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

IHC of mouse liver using anti-CD4 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD4 Ab at 1:750/DAB detection.

Western blot analysis of spleen cell line lysate using CD4 antibody.