CD4 antibody (FITC)

• Catalog Number: orb756848
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD4 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: EM4
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Normal human blood lymphocytes
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD4
• Entrez: 920
• UniProt ID: P01730
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-T4/Leu-3 antibody, anti- L3T4 antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human CD4 positive lymphocytes (red-filled) from CD4 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD4 (EM4) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (EM4) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).