CD4 antibody (FITC)

• Catalog Number: orb44809
• Category: Antibodies
• Description: Rat monoclonal antibody against CD4 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: GK1.5
• Tested applications: FC
• Reactivity: Mouse
• Isotype: Rat IgG2b
• Immunogen: Mouse CTL clone V4 cells
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-2 μg/ml.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD4
• Entrez: 12504
• UniProt ID: P06332
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) FITC antibody (concentration in sample 0.33 μg/ml).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) FITC antibody (concentration in sample 0.33 μg/ml).