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Catalog Number | orb382877 |
---|---|
Category | Proteins |
Description | CD3E protein |
Target | CD3E |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | 50mM Tris,50mM NaCl,pH8.0 |
Concentration | 0.5 mg/ml |
Purity | > 90% |
MW | 15.6 kDa |
Tested applications | ELISA, SDS-PAGE, WB |
Application notes | N-his6 |
Biological Origin | E.coli |
Storage | Store at -20°C for at least 1 year. After reconstitution, protein solution is stable at -20°C for 6 months to 12 months. Keep at -80°C for long term storage. Avoid repeated freeze/thaw cycles. |
Alternative names | FLJ18683 Proteins, T3E Proteins, TCRE Proteins, CD Read more... |
Note | For research use only |
NCBI | 000734 |
SDS-PAGE analysis of CD3E protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
11.9 kDa | |
Yeast |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation,the protein migrates to 44-46 & 46-48 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation,the protein migrates to 58-62 and 44-46 kDa based on Tris-Bis PAGE result. |