CD3E Antibody

• Catalog Number: orb1672178
• Category: Antibodies
• Description: CD3E Antibody
• Clonality: Recombinant
• Clone Number: YTH 12.5
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG lambda
• Immunogen: Human CD3.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD3E
• UniProt ID: P07766
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD3e, T-cell surface glycoprotein CD3 epsilon chai
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-CD3 antibody YTH 12.5. Rhesus monkey lymphocytes were stained with an isotype control (3.0- red) or the rmeric version of YTH 12.5 (orb1672178- blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analysed on a FlowJo single-cell flow cytometer.

Flow-cytometry using the anti-CD3E antibody YTH 12.5. Jurkat cells were stained with unimmunized r antibody (black line) or the rmeric version of YTH 12.5 (orb1672178 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Western Blot using anti-CD3E antibody YTH 12.5. Human spleen sample (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of YTH 12.5 (orb1672178) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for CD3E is 23.1 kDa. orb1672178 successfully detected human CD3E.

Immunohistochemical staining of human tonsil tissue using anti-CD3E antibody. YTH 12.5 Anti-CD3E staining of paraffin embedded human tissue using the rmeric version of YTH 12.5 (orb1672178). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1672178 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine- and counter-staining with haemotoxylin. Strong membrane staining of T-cells at the periphery of the germinal centre and between follicles may be observed. Recommended concentration- 2-4 ug/ml.

Flow-cytometry using anti-CD3 epsilon antibody YTH 12.5. Human lymphocytes were stained with an isotype control (3.0 - panel A) or the rmeric version of YTH 12.5 (orb1672178 - panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analysed on a FACSCanto flow-cytometer.