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Catalog Number | orb1252295 |
---|---|
Category | Antibodies |
Description | CD3E Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | C3e/1308 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody. |
Concentration | 0.2 mg/mL |
Dilution range | WB: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlOptimal dilution of the CD3e antibody should be determined by the researcher. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CD3E |
UniProt ID | P07766 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | T-cell surface glycoprotein CD3 epsilon chain, T-c Read more... |
Note | For research use only |
Application notes | WB: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlOptimal dilution of the CD3e antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC testing of human tonsil tissue with CD3e antibody (clone C3e/1308). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Western blot testing of 1) a partial recombinant protein and 2) human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.
SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone C3e/1308). Confirmation of Integrity and Purity of the Antibody.
ICC, IF, IHC-P, WB | |
Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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