CD3E Antibody

• Catalog Number: orb1252166
• Category: Antibodies
• Description: CD3E Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PC3/188A
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Isotype: IgG1
• Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlOptimal dilution of the CD3e antibody should be determined by the researcher.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD3E
• UniProt ID: P07766
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: T-cell surface glycoprotein CD3 epsilon chain, T-c
• Note: For research use only
• Application notes: WB: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlOptimal dilution of the CD3e antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.

SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.