CD3e antibody

• Catalog Number: orb1152492
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3e
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 898H2-6-15
• Tested applications: FC, IHC
• Reactivity: Porcine
• Isotype: IgG2a
• Immunogen: Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD3e
• UniProt ID: Q7YRN2
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leu4/T3; Cluster of Differentiation 3 epsilon chai
• Note: For research use only
• Application notes: The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.
• Expiration Date: 12 months from date of receipt.