CD3D Rabbit Polyclonal Antibody (Carrier-free)

SKU: orb3105772

Description

The CD3D Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, and WB applications. This antibody targets the T-cell surface glycoprotein CD3 delta chain in Human samples.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: Western blot, 0.2-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human CD3D recombinant protein (Position: F22-K171). Human CD3D shares 66% and 70.7% amino acid (aa) sequence identity with mouse and rat CD3D, respectively.
• Target: T-cell surface glycoprotein CD3 delta chain
• Molecular Weight: 19 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3 delta; CD3D; CD3-delta; T cell receptor T3 delta chain; T3D; TCRδ

Quick Database Links

Gene Symbol

T-cell surface glycoprotein CD3 delta chain

UniProt

P04234