CD36 Rabbit Polyclonal Antibody

• Catalog Number: orb10321
• Category: Antibodies
• Description: CD36 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, IP, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD36 (301-400/472aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1ug/Test, IP=1:20-50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 85-90 kDa
• Target: CD36
• UniProt ID: P16671
• RRID: AB_10751692
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Platelet glycoprotein 4; PAS-4; Platelet glycoprot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human HUVEC cell lysates, Lane 3: Human MCF-7cell lysates, Primary: Anti-CD36 (orb10321) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 90 kDa.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Raw264.7 cell lysates, Primary: Anti-CD36 (orb10321) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 100 kDa.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Raw264.7 cell lysates, Lane 3: Rat Placenta tissue lysates, Lane 4: Human HUVEC cell lysates, Lane 5: Human HL-60 cell lysates, Primary: Anti-CD36 (orb10321) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 100 kDa.

Tissue/cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CD36/PAS-4 Polyclonal Antibody, Unconjugated (orb10321) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.