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Catalog Number | orb371666 |
---|---|
Category | Antibodies |
Description | CD33 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human CD33 recombinant protein (Position: D18-H259). Human CD33 shares 61.6% amino acid (aa) sequence identity with mouse CD33. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Western blot, 0.1-0.5μg/mlFlow Cytometry, 1-3μg/1x106cells |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 39825 MW |
UniProt ID | P20138 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Myeloid cell surface antigen CD33;Sialic acid-bind Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-CD33 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CD33 using anti-CD33 antibody.Lane 1:SKOV3 cell.
IHC analysis of CD33 using anti-CD33 antibody. CD33 was detected in a paraffin-embedded section of human tonsil tissue.
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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