CD32 Rabbit Polyclonal Antibody

SKU: orb13406

Description

CD32 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=0.2μg/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Fc gamma RII a (201-300/317aa)
• Target: FCGR2A
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD32; CD32A; CDw32; FCG2; FCGR2; FCGR2A1; FcGR; FcgammaRIIa; IGFR2; CD32B; FCGR2C; FcGRIIB; FcRII-c; FcgammaRIIb; CD32C; FCRIIC; FcgammaRIIc; Fcgr3; Fcgr3a; fc-gamma RIII; fcRIII; FCRII; FcgammaRIIB2; FCG2A_HUMAN; FCGR2A; IgG Fc receptor II-a; Fc-gamma RII-a (Fc-gamma-RIIa | FcRII-a); FCG2B_HUMAN; FCGR2B; IgG Fc receptor II-b; Fc-gamma RII-b (Fc-gamma-RIIb | FcRII-b); FCG2C_HUMAN; IgG Fc receptor II-c; Fc-gamma RII-c (Fc-gamma-RIIc | FcRII-c); FCGR2_RAT; Fc-gamma RII (FcRII); IgG Fc receptor II beta;

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD32 antibody (orb13406), Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb13406, 0.2 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD32 (orb13406) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: K562 Cell Lysate at 30 ug, Primary: Anti-CD32 (orb13406) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 33 kD.

Quick Database Links

Gene Symbol

FCGR2A

UniProt

P12318