CD31/PECAM1 Antibody

• Catalog Number: orb196264
• Category: Antibodies
• Description: CD31/PECAM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 82536 MW
• UniProt ID: P16284
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Platelet endothelial cell adhesion molecule;PECAM-
• Note: For research use only
• Application notes: WB: The detection limit for CD31 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of Jurkat cells using anti-CD31/PECAM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody.Lane 1:human Jurkat cell;2:human Raji cell;3:human THP-1 cell.

IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody. CD31/PECAM1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue.

IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody. CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue.