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Catalog Number | orb1294369 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CD31 |
Target | CD31 / PECAM1 (Extracellular) |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit-IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human CD31 |
Tested applications | IHC, WB |
Dilution range | Western Blot 1:500-1:1000 Immunohistochemistry (Frozen) 1:100-1:200 Immunohistochemistry (Paraffin) 1:100-1:200 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Anti-CD31/PECAM1 antibody at 1/500 dilution, Lysates at 30 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294369 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling CD31 with orb1294369 at 1/100.
Immunohistochemical of frozen sections. Sample: liver. Green: CD31 (orb1294369): 1-100.
Anti-CD31/PECAM1 antibody at 1/500 dilution, Lysates at 30 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294369 overnight at 4°C.
Anti-CD31/PECAM1 antibody at 1/500 dilution, Lysates at 30 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294369 overnight at 4°C.