CD3 Rabbit Polyclonal Antibody

SKU: orb156305

Description

CD3 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Mouse
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from pig CD3 (11-100/196aa)
• Target: CD3E
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3epsilon; IMD18; T3E; TCRE; CD3; CD3E_HUMAN; CD3E; T-cell surface antigen T3/Leu-4 epsilon chain; CD3E_MOUSE; CD3 epsilon subunit of T-cell receptor complex; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e molecule; T-cell surface glycoprotein CD3 epsilon chain

Sample: Lane 1: Recombinant mouse CD3E protein, C-mFc (HEK293), Primary: Anti-CD3 (orb156305) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kDa, Observed band size: 36 kDa.

Quick Database Links

Gene Symbol

CD3E

Michinobu Ohno, Yasushi Fuchimoto, Huai-Che Hsu, Masataka Higuchi, Makoto Komura, Tetsuji Yamaoka, Akihiro Umezawa, Shin Enosawa, Tatsuo Kuroda Airway reconstruction using decellularized tracheal allografts in a porcine model Pediatric Surgery International, 33, 1065 (2017)

PubMed: 28819688

Applications

IHC

Reactivity

Porcine